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cell culture rat l6 skeletal muscle cell line  (ATCC)


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    ATCC cell culture rat l6 skeletal muscle cell line
    Effects of 5’-N-ethylcarboxamidoadenosine (NECA), 2-(4-[2-carboxyethyl]-phenethylamino) adenosine-52-nethyluronamide (CGS21680), and 8-[4-[4-(4-chlorophenzyl) piperazide-1-sulfonyl) phenyl]]-1-propylxanthine (PSB 603) on interleukin 6 (IL-6) messenger RNA (mRNA) gene expression in rat <t>L6</t> <t>skeletal</t> muscle myotubes using charcoal serum rat L6 skeletal muscle myotubes (70-90% confluent) were serum starved for 7 days and then stimulated for 1 hour IL-6 mRNA levels were measured relative to TATA-BOX using real-time quantitative polymerase chain reaction; stimulation was performed with vehicle (0.1% dimethyl sulphoxide), NECA (10 μM), PSB 603 (100 nM and 1 μM), CGS21680 (100 nM), and forskolin (100 nM), data were represented as means ± standard error of mean of at least three independent experimental groups *denotes p<0.05, **denotes p<0.01 and ***denotes p<0.001, data were analysed using a one-way ANOVA test followed by a Tukey test
    Cell Culture Rat L6 Skeletal Muscle Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1062 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cell culture rat l6 skeletal muscle cell line/product/ATCC
    Average 96 stars, based on 1062 article reviews
    cell culture rat l6 skeletal muscle cell line - by Bioz Stars, 2026-02
    96/100 stars

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    1) Product Images from "Adenosine A2B Receptors - Mediated Induction of Interleukin-6 in Skeletal Muscle Cells"

    Article Title: Adenosine A2B Receptors - Mediated Induction of Interleukin-6 in Skeletal Muscle Cells

    Journal: Turkish Journal of Pharmaceutical Sciences

    doi: 10.4274/tjps.08108

    Effects of 5’-N-ethylcarboxamidoadenosine (NECA), 2-(4-[2-carboxyethyl]-phenethylamino) adenosine-52-nethyluronamide (CGS21680), and 8-[4-[4-(4-chlorophenzyl) piperazide-1-sulfonyl) phenyl]]-1-propylxanthine (PSB 603) on interleukin 6 (IL-6) messenger RNA (mRNA) gene expression in rat L6 skeletal muscle myotubes using charcoal serum rat L6 skeletal muscle myotubes (70-90% confluent) were serum starved for 7 days and then stimulated for 1 hour IL-6 mRNA levels were measured relative to TATA-BOX using real-time quantitative polymerase chain reaction; stimulation was performed with vehicle (0.1% dimethyl sulphoxide), NECA (10 μM), PSB 603 (100 nM and 1 μM), CGS21680 (100 nM), and forskolin (100 nM), data were represented as means ± standard error of mean of at least three independent experimental groups *denotes p<0.05, **denotes p<0.01 and ***denotes p<0.001, data were analysed using a one-way ANOVA test followed by a Tukey test
    Figure Legend Snippet: Effects of 5’-N-ethylcarboxamidoadenosine (NECA), 2-(4-[2-carboxyethyl]-phenethylamino) adenosine-52-nethyluronamide (CGS21680), and 8-[4-[4-(4-chlorophenzyl) piperazide-1-sulfonyl) phenyl]]-1-propylxanthine (PSB 603) on interleukin 6 (IL-6) messenger RNA (mRNA) gene expression in rat L6 skeletal muscle myotubes using charcoal serum rat L6 skeletal muscle myotubes (70-90% confluent) were serum starved for 7 days and then stimulated for 1 hour IL-6 mRNA levels were measured relative to TATA-BOX using real-time quantitative polymerase chain reaction; stimulation was performed with vehicle (0.1% dimethyl sulphoxide), NECA (10 μM), PSB 603 (100 nM and 1 μM), CGS21680 (100 nM), and forskolin (100 nM), data were represented as means ± standard error of mean of at least three independent experimental groups *denotes p<0.05, **denotes p<0.01 and ***denotes p<0.001, data were analysed using a one-way ANOVA test followed by a Tukey test

    Techniques Used: Gene Expression, Real-time Polymerase Chain Reaction

    Effects of 5’-N-ethylcarboxamidoadenosine (NECA), 2-(4-[2-carboxyethyl]-phenethylamino) adenosine-52-nethyluronamide (CGS21680) and 8-[4-[4-(4-chlorophenzyl) piperazide-1-sulfonyl) phenyl]]-1-propylxanthine (PSB 603) on interleukin 6 (IL-6) messenger RNA (mRNA) gene expression in rat L6 skeletal muscle myotubes using charcoal serum, rat L6 skeletal muscle myotubes were stimulated for the indicated time from 1 hour to 24 hours and interleukin-6 mRNA levels, relative to TATA-BOX, was measured by quantitative real time polymerase chain reaction; stimulation was performed with vehicle (0.1% dimethyl sulphoxide), NECA (100 nM and 10 μM), PSB 603 (10 μM), CGS21680 (100 nM) and forskolin (1 μM) (a) Stimulation was performed up to 1 hour (b) Stimulation was performed for up to 3 hours (c) Stimulation was performed for up to 24 hours, data were represented as means ± standard error of mean of three independent experimental group (n=3; *denotes p<0.05, **denotes p<0.01 and ***denotes p<0.001), data were analyzed using one way ANOVA test followed by Tukey test
    Figure Legend Snippet: Effects of 5’-N-ethylcarboxamidoadenosine (NECA), 2-(4-[2-carboxyethyl]-phenethylamino) adenosine-52-nethyluronamide (CGS21680) and 8-[4-[4-(4-chlorophenzyl) piperazide-1-sulfonyl) phenyl]]-1-propylxanthine (PSB 603) on interleukin 6 (IL-6) messenger RNA (mRNA) gene expression in rat L6 skeletal muscle myotubes using charcoal serum, rat L6 skeletal muscle myotubes were stimulated for the indicated time from 1 hour to 24 hours and interleukin-6 mRNA levels, relative to TATA-BOX, was measured by quantitative real time polymerase chain reaction; stimulation was performed with vehicle (0.1% dimethyl sulphoxide), NECA (100 nM and 10 μM), PSB 603 (10 μM), CGS21680 (100 nM) and forskolin (1 μM) (a) Stimulation was performed up to 1 hour (b) Stimulation was performed for up to 3 hours (c) Stimulation was performed for up to 24 hours, data were represented as means ± standard error of mean of three independent experimental group (n=3; *denotes p<0.05, **denotes p<0.01 and ***denotes p<0.001), data were analyzed using one way ANOVA test followed by Tukey test

    Techniques Used: Gene Expression, Real-time Polymerase Chain Reaction



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    cell culture rat l6 skeletal muscle cell line  (ATCC)
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    ATCC cell culture rat l6 skeletal muscle cell line
    Effects of 5’-N-ethylcarboxamidoadenosine (NECA), 2-(4-[2-carboxyethyl]-phenethylamino) adenosine-52-nethyluronamide (CGS21680), and 8-[4-[4-(4-chlorophenzyl) piperazide-1-sulfonyl) phenyl]]-1-propylxanthine (PSB 603) on interleukin 6 (IL-6) messenger RNA (mRNA) gene expression in rat <t>L6</t> <t>skeletal</t> muscle myotubes using charcoal serum rat L6 skeletal muscle myotubes (70-90% confluent) were serum starved for 7 days and then stimulated for 1 hour IL-6 mRNA levels were measured relative to TATA-BOX using real-time quantitative polymerase chain reaction; stimulation was performed with vehicle (0.1% dimethyl sulphoxide), NECA (10 μM), PSB 603 (100 nM and 1 μM), CGS21680 (100 nM), and forskolin (100 nM), data were represented as means ± standard error of mean of at least three independent experimental groups *denotes p<0.05, **denotes p<0.01 and ***denotes p<0.001, data were analysed using a one-way ANOVA test followed by a Tukey test
    Cell Culture Rat L6 Skeletal Muscle Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cell culture rat l6 skeletal muscle cell line/product/ATCC
    Average 96 stars, based on 1 article reviews
    cell culture rat l6 skeletal muscle cell line - by Bioz Stars, 2026-02
    96/100 stars
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    cell culture rat l6 skeletal muscle cell line crl  (ATCC)
    96
    ATCC cell culture rat l6 skeletal muscle cell line crl
    Effects of 5’-N-ethylcarboxamidoadenosine (NECA), 2-(4-[2-carboxyethyl]-phenethylamino) adenosine-52-nethyluronamide (CGS21680), and 8-[4-[4-(4-chlorophenzyl) piperazide-1-sulfonyl) phenyl]]-1-propylxanthine (PSB 603) on interleukin 6 (IL-6) messenger RNA (mRNA) gene expression in rat <t>L6</t> <t>skeletal</t> muscle myotubes using charcoal serum rat L6 skeletal muscle myotubes (70-90% confluent) were serum starved for 7 days and then stimulated for 1 hour IL-6 mRNA levels were measured relative to TATA-BOX using real-time quantitative polymerase chain reaction; stimulation was performed with vehicle (0.1% dimethyl sulphoxide), NECA (10 μM), PSB 603 (100 nM and 1 μM), CGS21680 (100 nM), and forskolin (100 nM), data were represented as means ± standard error of mean of at least three independent experimental groups *denotes p<0.05, **denotes p<0.01 and ***denotes p<0.001, data were analysed using a one-way ANOVA test followed by a Tukey test
    Cell Culture Rat L6 Skeletal Muscle Cell Line Crl, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cell culture rat l6 skeletal muscle cell line crl/product/ATCC
    Average 96 stars, based on 1 article reviews
    cell culture rat l6 skeletal muscle cell line crl - by Bioz Stars, 2026-02
    96/100 stars
    		  Buy from Supplier

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    Effects of 5’-N-ethylcarboxamidoadenosine (NECA), 2-(4-[2-carboxyethyl]-phenethylamino) adenosine-52-nethyluronamide (CGS21680), and 8-[4-[4-(4-chlorophenzyl) piperazide-1-sulfonyl) phenyl]]-1-propylxanthine (PSB 603) on interleukin 6 (IL-6) messenger RNA (mRNA) gene expression in rat L6 skeletal muscle myotubes using charcoal serum rat L6 skeletal muscle myotubes (70-90% confluent) were serum starved for 7 days and then stimulated for 1 hour IL-6 mRNA levels were measured relative to TATA-BOX using real-time quantitative polymerase chain reaction; stimulation was performed with vehicle (0.1% dimethyl sulphoxide), NECA (10 μM), PSB 603 (100 nM and 1 μM), CGS21680 (100 nM), and forskolin (100 nM), data were represented as means ± standard error of mean of at least three independent experimental groups *denotes p<0.05, **denotes p<0.01 and ***denotes p<0.001, data were analysed using a one-way ANOVA test followed by a Tukey test

    Journal: Turkish Journal of Pharmaceutical Sciences

    Article Title: Adenosine A2B Receptors - Mediated Induction of Interleukin-6 in Skeletal Muscle Cells

    doi: 10.4274/tjps.08108

    Figure Lengend Snippet: Effects of 5’-N-ethylcarboxamidoadenosine (NECA), 2-(4-[2-carboxyethyl]-phenethylamino) adenosine-52-nethyluronamide (CGS21680), and 8-[4-[4-(4-chlorophenzyl) piperazide-1-sulfonyl) phenyl]]-1-propylxanthine (PSB 603) on interleukin 6 (IL-6) messenger RNA (mRNA) gene expression in rat L6 skeletal muscle myotubes using charcoal serum rat L6 skeletal muscle myotubes (70-90% confluent) were serum starved for 7 days and then stimulated for 1 hour IL-6 mRNA levels were measured relative to TATA-BOX using real-time quantitative polymerase chain reaction; stimulation was performed with vehicle (0.1% dimethyl sulphoxide), NECA (10 μM), PSB 603 (100 nM and 1 μM), CGS21680 (100 nM), and forskolin (100 nM), data were represented as means ± standard error of mean of at least three independent experimental groups *denotes p<0.05, **denotes p<0.01 and ***denotes p<0.001, data were analysed using a one-way ANOVA test followed by a Tukey test

    Article Snippet: Cell culture Rat L6 skeletal muscle cell line and myoblast cell line were originally obtained from the American Type Culture Collection (USA).

    Techniques: Gene Expression, Real-time Polymerase Chain Reaction

    Effects of 5’-N-ethylcarboxamidoadenosine (NECA), 2-(4-[2-carboxyethyl]-phenethylamino) adenosine-52-nethyluronamide (CGS21680) and 8-[4-[4-(4-chlorophenzyl) piperazide-1-sulfonyl) phenyl]]-1-propylxanthine (PSB 603) on interleukin 6 (IL-6) messenger RNA (mRNA) gene expression in rat L6 skeletal muscle myotubes using charcoal serum, rat L6 skeletal muscle myotubes were stimulated for the indicated time from 1 hour to 24 hours and interleukin-6 mRNA levels, relative to TATA-BOX, was measured by quantitative real time polymerase chain reaction; stimulation was performed with vehicle (0.1% dimethyl sulphoxide), NECA (100 nM and 10 μM), PSB 603 (10 μM), CGS21680 (100 nM) and forskolin (1 μM) (a) Stimulation was performed up to 1 hour (b) Stimulation was performed for up to 3 hours (c) Stimulation was performed for up to 24 hours, data were represented as means ± standard error of mean of three independent experimental group (n=3; *denotes p<0.05, **denotes p<0.01 and ***denotes p<0.001), data were analyzed using one way ANOVA test followed by Tukey test

    Journal: Turkish Journal of Pharmaceutical Sciences

    Article Title: Adenosine A2B Receptors - Mediated Induction of Interleukin-6 in Skeletal Muscle Cells

    doi: 10.4274/tjps.08108

    Figure Lengend Snippet: Effects of 5’-N-ethylcarboxamidoadenosine (NECA), 2-(4-[2-carboxyethyl]-phenethylamino) adenosine-52-nethyluronamide (CGS21680) and 8-[4-[4-(4-chlorophenzyl) piperazide-1-sulfonyl) phenyl]]-1-propylxanthine (PSB 603) on interleukin 6 (IL-6) messenger RNA (mRNA) gene expression in rat L6 skeletal muscle myotubes using charcoal serum, rat L6 skeletal muscle myotubes were stimulated for the indicated time from 1 hour to 24 hours and interleukin-6 mRNA levels, relative to TATA-BOX, was measured by quantitative real time polymerase chain reaction; stimulation was performed with vehicle (0.1% dimethyl sulphoxide), NECA (100 nM and 10 μM), PSB 603 (10 μM), CGS21680 (100 nM) and forskolin (1 μM) (a) Stimulation was performed up to 1 hour (b) Stimulation was performed for up to 3 hours (c) Stimulation was performed for up to 24 hours, data were represented as means ± standard error of mean of three independent experimental group (n=3; *denotes p<0.05, **denotes p<0.01 and ***denotes p<0.001), data were analyzed using one way ANOVA test followed by Tukey test

    Article Snippet: Cell culture Rat L6 skeletal muscle cell line and myoblast cell line were originally obtained from the American Type Culture Collection (USA).

    Techniques: Gene Expression, Real-time Polymerase Chain Reaction